Regulation of proto-oncogene transcription, cell proliferation, and tumorigenesis in mice by PSF protein and a VL30 noncoding RNA

• DOI: 10.1073/pnas.0909022106
• Publication Date: 2009-09-12T01:45:19Z
• Authors: Gang Wang; Ying Cui; Guangfeng Zhang; Alan Garen; Xu Song
• Keywords: Transcription

We describe the role of PSF protein and VL30–1 RNA, a mouse retroelement noncoding in reversible regulation proto-oncogene transcription, cell proliferation, tumorigenesis mice. The experiments involved increasing expression or RNA NIH/3T3 fibroblast cells B16F10 melanoma by transfecting respective coding genes under control strong promoter decreasing shRNA construct that causes degradation mRNA RNA. results are as follows: ( i ) binds to Rab23 , repressing releases from activating transcription; ii suppresses proliferation culture mice; iii promotes These indicate is major tumor-suppressor tumor-promoter Although can integrate into genome, tumor promotion involves trans effect rather than cis on gene transcription. Expression 5- 8-fold higher lines myoblast lines, whereas does not decrease suggesting driven an increase PSF. A similar regulatory mechanism functions human cells, except PSF-binding RNAs replace which encoded genome. propose have central mammalian potential therapeutic strategy for cancer.