Two physically, functionally, and developmentally distinct peritoneal macrophage subsets

• DOI: 10.1073/pnas.0915000107
• Publication Date: 2010-01-26T02:54:47Z
• Authors: Eliver Eid Bou Ghosn; Alexandra A. Cassado; Gregory R. Govoni; Takeshi Fukuhara; Yang Yang; Denise M. Monack; Karina R. Bortoluci; Sandro R. Almeida; Leonard A. Herzen...; Leonore A. Herzen...
• Keywords: Peritoneal cavity; Ex vivo

The peritoneal cavity (PerC) is a unique compartment within which variety of immune cells reside, and from macrophages (MØ) are commonly drawn for functional studies. Here we define two MØ subsets that coexist in PerC adult mice. One, provisionally called the large (LPM), contains approximately 90% unstimulated animals but disappears rapidly following lipopolysaccharide (LPS) or thioglycolate stimulation. These express high levels canonical surface markers, CD11b F4/80. second subset, referred to as small (SPM), expresses substantially lower F4/80 MHC-II, not expressed on LPM. SPM, predominates after LPS stimulation, does derive Instead, it derives blood monocytes enter stimulation differentiate mature SPM 2 4 d. Both show clear phagocytic activity both produce nitric oxide (NO) response vivo. However, their responses key differences: vitro, stimulates LPM, NO; vivo, NO, albeit with different patterns. findings extend current models heterogeneity shed new light diversity, development, function. Thus, they introduce context interpreting (and reinterpreting) data ex vivo studies MØ.