Vertebrate-specific glutaredoxin is essential for brain development

• DOI: 10.1073/pnas.1110085108
• Publication Date: 2011-12-03T20:27:25Z
• Authors: Lars Bräutigam; Lena Dorothee Sch...; José Rodrigo Godoy; Timour Prozorovski; Manuela Gellert; Giselbert Hauptmann; Arne Holmgren; Christopher Horst...; Carsten Berndt
• Keywords: Glutaredoxin; Cell fate determination; Thiol

Cellular functions and survival are dependent on a tightly controlled redox potential. Currently, an increasing amount of data supports the concept local changes in environment specific signaling events controlling cell function. Specific protein thiol groups major targets regulation. Thioredoxins glutaredoxins catalyze reversible thiol-disulfide exchange reactions primary regulators state. Here, we demonstrate that embryonic brain development depends enzymatic activity glutaredoxin 2. Zebrafish with silenced expression 2 lost virtually all types neurons by apoptotic death ability to develop axonal scaffold. As demonstrated zebrafish human cellular model for neuronal differentiation, controls outgrowth via regulation collapsin response mediator 2, central component semaphorin pathway. This study provides example essential vertebrate development.