Arabidopsis lysin-motif proteins LYM1 LYM3 CERK1 mediate bacterial peptidoglycan sensing and immunity to bacterial infection

• DOI: 10.1073/pnas.1112862108
• Publication Date: 2011-11-22T07:22:31Z
• Authors: Roland Willmann; Heini M. Lajunen; Gitte Erbs; Mari-Anne Newman; Dagmar Kolb; Kenichi Tsuda; Fumiaki Katagiri; Judith Fliegmann; Jean-Jacques Bono; Julie V. Cullimore; Anna K. Jehle; Friedrich Götz; Andreas Kulik; Antonio Molinaro; Volker Lipka; Andrea A. Gust; Thorsten Nürnberger
• Keywords: Pseudomonas syringae; Lysin

Recognition of microbial patterns by host pattern recognition receptors is a key step in immune activation multicellular eukaryotes. Peptidoglycans (PGNs) are major components bacterial cell walls that possess immunity-stimulating activities metazoans and plants. Here we show PGN sensing immunity to infection Arabidopsis thaliana requires three lysin-motif (LysM) domain proteins. LYM1 LYM3 plasma membrane proteins physically interact with PGNs mediate sensitivity structurally different from Gram-negative Gram-positive bacteria. lym1 lym3 mutants lack PGN-induced changes transcriptome activity patterns, but respond fungus-derived chitin, related PGNs, wild-type manner. Notably, , mutant genotypes exhibit supersusceptibility virulent Pseudomonas syringae pathovar tomato DC3000. Defects basal double resemble those observed single mutants, suggesting both part the same system. We further deletion CERK1, LysM receptor kinase had previously been implicated chitin perception fungal phenocopies defects such as peptidoglycan insensitivity enhanced susceptibility infection. Altogether, our findings suggest plants share ability recognize PGNs. However, LYM1, LYM3, CERK1 form system unrelated metazoan receptors, propose lineage-specific systems have arisen through convergent evolution.