Interrogating translational efficiency and lineage-specific transcriptomes using ribosome affinity purification

• DOI: 10.1073/pnas.1304124110
• Publication Date: 2013-09-04T01:58:16Z
• Authors: Pingzhu Zhou; Yijing Zhang; Qing Ma; Fei Gu; Daniel S. Day; Aibin He; Bin Zhou; Jing Li; Sean M. Stevens; Daniel Romo; William T. Pu
• Keywords: Ribosome profiling; Ribosomal binding site; Translational efficiency; Lineage (genetic); Translational regulation

Significance We developed reagents and approaches to pull down ribosome-associated RNAs from Cre-labeled cells. show that this strategy is useful probe cell type-specific gene expression the extent of transcript binding ribosomes.