Microenvironmental reprogramming by three-dimensional culture enables dermal papilla cells to induce de novo human hair-follicle growth

• DOI: 10.1073/pnas.1309970110
• Publication Date: 2013-10-22T02:57:55Z
• Authors: Claire A. Higgins; James C. Chen; Jane E. Cerise; Colin A. B. Jahoda; Angela M. Christiano
• Keywords: Dermal papillae; Neogenesis; Epidermis (zoology); Reprogramming; Human skin

Significance Growth of de novo hair follicles in adult skin occurs by a process known as neogenesis. One way initiating neogenesis is to place dermal papillae isolated from the follicle contact with an overlying epidermis where they reprogram adopt follicular fate. This approach, however, has not been successful using cultured human papilla cells because lose their ability induce growth after expansion vitro. In this paper, we demonstrate that manipulating cell culture conditions establish three-dimensional spheroids, restore inductivity. We also use several systems biology approaches gain comprehensive understanding molecular mechanisms underlie regenerative process.