High-resolution restoration of 3D structures from widefield images with extreme low signal-to-noise-ratio

• DOI: 10.1073/pnas.1315675110
• Publication Date: 2013-10-09T05:43:51Z
• Authors: Muthuvel Arigovindan; Jennifer C. Fung; Daniel Elnatan; Vito Mennella; Yee-Hung Mark Chan; Michael Pollard; Eric Branlund; John W. Sedat; David A. Agard
• Keywords: Photobleaching; Point spread function

Significance Recording 3D fluorescent movies has become a critical tool of modern cell biology. Unfortunately, this requires exposure the sample to such significant amounts illumination light that fluorophores photobleached and resultant oxygen radicals can significantly perturb cellular function (phototoxicity). Although widefield microscopy is very efficient, generating high-quality reconstructions removal out-of-focus in process called deconvolution. most deconvolution methods require high signal-to-noise ratios are thus incompatible with low levels required for unperturbed vivo imaging. Here we present novel method solves problem, allowing be reduced extremely levels, resulting an enabling technology