Self-organization of axial polarity, inside-out layer pattern, and species-specific progenitor dynamics in human ES cell–derived neocortex

• DOI: 10.1073/pnas.1315710110
• Publication Date: 2013-11-26T00:14:48Z
• Authors: Taisuke Kadoshima; Hideya Sakaguchi; Tokushige Nakano; Mika Soen; Satoshi Ando; Mototsugu Eiraku; Yoshiki Sasai
• Keywords: 0303 health sciences; Pyridines; Organogenesis; Cell Culture Techniques; Cell Polarity; Neocortex; Amides; Drug Combinations; 03 medical and health sciences; Species Specificity; Humans; Proteoglycans; Collagen; Laminin; Neuroglia; Embryonic Stem Cells

Significance Using 3D culture of human ES cells, we show new self-organizing aspects of human corticogenesis: spontaneous development of intracortical polarity, curving morphology, and complex zone separations. Moreover, this culture generates species-specific progenitors, outer radial glia, which are abundantly present in the human, but not mouse, neocortex. Our study suggests an unexpectedly wide range of self-organizing events that are driven by internal programs in human neocortex development.