The endocannabinoid 2-AG controls skeletal muscle cell differentiation via CB1 receptor-dependent inhibition of Kv7 channels

• DOI: 10.1073/pnas.1406728111
• Publication Date: 2014-06-04T03:26:28Z
• Authors: Fabio A. Iannotti; Cristoforo Silvestri; Enrico Mazzarella; Andrea Martella; Daniela Calvigioni; Fabiana Piscitelli; Paolo Ambrosino; Stefania Petrosino; Gabriella Czifra; Tamás Bíró; Tibor Harkany; Maurizio Tagliala...; Vincenzo Di Marzo
• Keywords: 0301 basic medicine; KCNQ Potassium Channels; Inositol Phosphates; Myoblasts, Skeletal; Muscle Fibers, Skeletal; Cell Differentiation; Orvostudományok; Arachidonic Acids; CHO Cells; Muscle Development; Glycerides; Receptor, Cannabinoid, CB2; Mice; 03 medical and health sciences; Cricetulus; Receptor, Cannabinoid, CB1; Cricetinae; Animals; Humans; Elméleti orvostudományok; Gene Silencing; Muscle, Skeletal; Cell Proliferation; Endocannabinoids

Significance Although CB1 cannabinoid receptors control skeletal muscle insulin signaling, little is known of their role in formation during differentiation from myoblasts to myotubes. The voltage-dependent K v 7 + channels, which are tonically activated by the membrane phospholipid phosphatidylinositol 4,5-bisphosphate (PIP2), instead activate myotube formation. We found that levels endogenous agonist 2-arachidonoylglycerol decreased murine myoblast into myotubes, whereas expression up-regulated. activation inhibits This effect exerted reducing PIP2 binding 7.4, represents subunit responsible for pro-myogenic effects. Accordingly, 7.4-mediated currents transfected CHO cells. endocannabinoid system might thus play a dystrophies.