Ghrelin receptor conformational dynamics regulate the transition from a preassembled to an active receptor:Gq complex

• DOI: 10.1073/pnas.1414618112
• Publication Date: 2015-01-21T06:19:22Z
• Authors: Marjorie Damian; Sophie Mary; Mathieu Maingot; Céline M'Kadmi; Didier Gagne; Jean-Philippe Leyris; Séverine Denoyelle; Gérald Gaibelet; Laurent Gavara; Mauricio Garcia d...; David Perahia; Eric Trinquet; Bernard Mouillac; Ségolène Galandrin; Céline Galès; Jean-Alain Fehrentz; Nicolas Floquet; Jean Martinez; Jacky Marie; Jean-Louis Banères
• Keywords: Gq alpha subunit; Cell surface receptor

Significance G protein-coupled receptors (GPCRs), one of the largest cell surface receptor families, transmit their signals through coupling intracellular partners, such as proteins. Knowing how this occurs is essential, because it governs entire signaling process. To address open question, we used a purified GPCR model to which applied various state-of-the-art biochemical and biophysical approaches. By doing so, provide direct experimental evidence mechanism in conformational changes are directly linked rearrangement preassembled complex between its cognate Gq protein. This sheds light on way interacts with proteins trigger signaling.