Tetrahydrobiopterin and alkylglycerol monooxygenase substantially alter the murine macrophage lipidome

• DOI: 10.1073/pnas.1414887112
• Publication Date: 2015-02-10T04:13:27Z
• Authors: Katrin Watschinger; Markus A. Keller; Eileen McNeill; Mohammad T. Alam; Steven Lai; Sabrina Sailer; Veronika Rauch; Jyoti Patel; Albin Hermetter; Georg Golderer; Stephan Geley; Gabriele Werner-F...; Robert S. Plumb; Giuseppe Astarita; Markus Ralser; Keith M. Channon; Ernst R. Werner
• Keywords: Lipidome; Biopterin; NOS1

Significance We have recently identified the sequence of ether lipid-cleaving enzyme alkylglycerol monooxygenase. Like nitric oxide synthases and aromatic amino acid hydroxylases, monooxygenase needs tetrahydrobiopterin as cofactor. Whereas former enzymes well-established roles in cell, physiology is still not clear. Here we show its regulation murine macrophage differentiation dependence on cofactor live macrophage-like RAW264.7 cells. Upon modulation activity key biosynthesis, observe extensive changes various lipid classes ranging from lipids to far more complex lipids. These findings point an important role cellular homeostasis.