Regulation by a chaperone improves substrate selectivity during cotranslational protein targeting

• DOI: 10.1073/pnas.1422594112
• Publication Date: 2015-06-10T17:23:59Z
• Authors: Aileen Ariosa; Jae Ho Lee; Shuai Wang; Ishu Saraogi; Shu-ou Shan
• Keywords: Signal recognition particle; Chaperone (clinical); ribosome biogenesis

Significance Correct protein biogenesis is crucial for all cells. Numerous factors including molecular chaperones, modification enzymes, and protein-targeting machineries bind near the ribosome exit site can access nascent protein. How proteins are accurately selected into correct pathway in such a crowded environment an emerging question central to accurate biogenesis. Using chemical biology biochemical biophysical tools, we show that major cotranslational chaperone, trigger factor, targeting machinery, signal recognition particle, regulate each other at multiple stages, initial binding, delivery membrane, enforcement of timer targeting. Together, these mechanisms enhance substrate selection both posttranslational pathways.