Transcription factor p63 controls the reserve status but not the stemness of horizontal basal cells in the olfactory epithelium

• DOI: 10.1073/pnas.1512272112
• Publication Date: 2015-08-26T01:44:40Z
• Authors: Nikolai Schnittke; Daniel B. Herrick; Brian Lin; Jesse Peterson; Julie H. Coleman; Adam I. Packard; Woochan Jang; James E. Schwob
• Keywords: Multipotent Stem Cell; Developmental Biology

Adult tissue stem cells can serve two broad functions: to participate actively in the maintenance and regeneration of a or wait reserve only when activated from dormant state. The adult olfactory epithelium, site for ongoing, life-long, robust neurogenesis, contains both these functional cell types. Globose basal (GBCs) act as active population give rise all differentiated found normal tissue. Horizontal (HBCs) remain unless by injury. Here we show that HBC activation following injury olfactotoxic gas methyl bromide is coincident with down-regulation protein 63 (p63) but anticipates proliferation. Gain- loss-of-function studies this p63 necessary sufficient activation. Moreover, HBCs GBCs persist months continue bona fide participating over long term. Our analysis provides mechanistic insight into dynamics between subtypes demonstrates regulates state not status HBCs.