PPR-SMR protein SOT1 has RNA endonuclease activity

• DOI: 10.1073/pnas.1612460114
• Publication Date: 2017-02-07T02:05:30Z
• Authors: Wen Zhou; Qingtao Lu; Qingwei Li; Lei Wang; Shunhua Ding; Aihong Zhang; Xiaogang Wen; Lixin Zhang; Congming Lu
• Keywords: Pentatricopeptide repeat; 23S ribosomal RNA

Significance Our results demonstrate that SUPPRESSOR OF THYLAKOID FORMATION 1 (SOT1), an Arabidopsis pentatricopeptide repeat (PPR) protein with a small MutS-related (SMR) domain, has endonuclease activity. The SMR moiety of SOT1 performs the endonucleolytic maturation 23S and 4.5S rRNA through PPR domain specifically recognizing 13-nucleotide RNA sequence in 5′ end chloroplast 23S–4.5S precursor. also show can be engineered to recognize cleave predicted substrate. findings suggest could used as tool for manipulation future.