Comprehensive mapping of abiotic stress inputs into the soybean circadian clock

• DOI: 10.1073/pnas.1708508116
• Publication Date: 2019-11-01T23:49:06Z
• Authors: Meina Li; Lijun Cao; Musoki Mwimba; Yan Zhou; Ling Li; Mian Zhou; Patrick S. Schnable; Jamie A. O’Rourke; Xinnian Dong; Wei Wang
• Keywords: Plant Leaves; 2. Zero hunger; 0303 health sciences; 03 medical and health sciences; Glycine max; Stress, Physiological; Circadian Clocks; Arabidopsis; Biological Sciences; 15. Life on land; Genes, Plant

The plant circadian clock evolved to increase fitness by synchronizing physiological processes with environmental oscillations. Crop was artificially selected through domestication and breeding, the identified both natural artificial selections as a key improved fitness. Despite progress in Arabidopsis , our understanding of crop is still limited, impeding its rational improvement for enhanced To unveil interactions between various cues, we comprehensively mapped abiotic stress inputs soybean using 2-module discovery pipeline. Using “molecular timetable” method, computationally surveyed publicly available stress-related transcriptomes identify stresses that have strong impacts on global rhythm. These findings were then experimentally confirmed multiplexed RNA sequencing technology. Specific components modulated each further identified. This comprehensive mapping uncovered such alkaline stress. Moreover, short-term iron deficiency targeted different thus had opposite effects clocks these 2 species. Comparing varieties uptake efficiencies suggests phase modulation might be mechanism alleviate symptoms soybean. unique responses demonstrate need directly study clocks. Our pipeline may serve broadly applicable tool facilitate explorations.