PRDM1 silences stem cell-related genes and inhibits proliferation of human colon tumor organoids

• DOI: 10.1073/pnas.1802902115
• Publication Date: 2018-05-14T19:21:23Z
• Authors: Changlong Liu; Carolyn E. Banister; Charles C. Weige; Diego Altomare; Joseph H. Richardson; Carlo M. Contreras; Phillip J. Buckha...
• Keywords: Organoid; LGR5

PRDM1 is a tumor suppressor that plays an important role in B and T cell lymphomas. Our previous studies demonstrated PRDM1β p53-response gene human colorectal cancer cells. However, the function of cells colon organoids not clear. Here we show low expression predicts poor survival patients. We engineered knockouts overexpression clones RKO characterized PRDM1-dependent transcript landscapes, revealing both α β isoforms repress MYC-response genes stem cell-related genes. Finally, forced prevents formation growth vitro. These results suggest p53 may exert tumor-suppressive effects part through silencing genes, depleting size normal intestinal compartment response to DNA damage.