Genome-wide regulation of light-controlled seedling morphogenesis by three families of transcription factors

• DOI: 10.1073/pnas.1803861115
• Publication Date: 2018-05-29T15:35:52Z
• Authors: Hui Shi; Mohan Lyu; Yiwen Luo; Shoucheng Liu; Yue Li; Hang He; Ning Wei; Xing Wang Deng; Shangwei Zhong
• Keywords: Transcription

Three families of transcription factors have been reported to play key roles in light control Arabidopsis seedling morphogenesis. Among them, bHLH protein PIFs and plant-specific EIN3/EIN3-LIKE 1 (EIN3/EIL1) accumulate the dark maintain skotomorphogenesis. On other hand, HY5 HOMOLOG (HYH), two related bZIP proteins, are stabilized promote photomorphogenic development. To systemically investigate transcriptional regulation light-controlled morphogenesis, we generated HY5ox/pifQein3eil1, which contained mutations EIN3/EIL1 four PIF genes (pifQein3eil1) overexpression Our results show that dark-grown HY5ox/pifQein3eil1 seedlings display a photomorphogenesis highly similar wild-type grown continuous light, with remarkably enhanced phenotypes compared pifQ mutants. Consistent genetic evidence, transcriptome analysis indicated PIFs, EIN3/EIL1, dominant collectively mediating wide range light-caused genome-wide changes. Moreover, independently expression light-regulated such as HLS1 cooperatively regulate apical hook formation, hypocotyl elongation, cotyledon opening expansion. This study illustrates comprehensive regulatory network activities correspond specific morphological aspects skotomorphogenesis photomorphogenesis.