Histone 2B monoubiquitination complex integrates transcript elongation with RNA processing at circadian clock and flowering regulators

• DOI: 10.1073/pnas.1806541116
• Publication Date: 2019-03-29T06:54:12Z
• Authors: Magdalena Woloszy...; Sabine Le Gall; Pia Neyt; Tommaso M. Boccardi; Marion Grasser; Gernot Längst; Stijn Aesaert; Griet Coussens; Stijn Dhondt; Eveline Van De Sl...; Leonardo Bruno; Jorge Fung-Uceda; Paloma Mas; Marc Van Montagu; Dirk Inzé; Kristiina Himanen; Geert De Jaeger; Klaus D. Grasser; Mieke Van Lijsebe...
• Keywords: Flowering Locus C

HISTONE MONOUBIQUITINATION1 (HUB1) and its paralog HUB2 act in a conserved heterotetrameric complex the chromatin-mediated transcriptional modulation of developmental programs, such as flowering time, dormancy, circadian clock. The KHD1 SPEN3 proteins were identified interactors HUB1 with vitro RNA-binding activity. Mutants had reduced rosette leaf areas. Strikingly, spen3 mutants, time was slightly, but significantly, delayed, opposed to early hub1-4 mutant. mutant phenotypes biomass suggested deregulation their respective regulatory genes CIRCADIAN CLOCK-ASSOCIATED1 ( CCA1 ) FLOWERING LOCUS C FLC that are known targets HUB1-mediated histone H2B monoubiquitination (H2Bub). Indeed, spen3-1 clock period shortened observed by luciferase reporter assays, levels α β splice forms altered, expression H2Bub reduced. In mutant, delay correlated an enhanced expression, possibly due increased distal versus proximal ratio antisense COOLAIR transcript. Together transcriptomic double-mutant analyses, our data revealed interaction links during transcript elongation pre-mRNA processing at . Furthermore, presence intact is required for function formation -derived transcripts.