A molecular profile of a hematopoietic stem cell niche

• DOI: 10.1073/pnas.192124499
• Publication Date: 2002-10-01T16:58:07Z
• Authors: Jason A. Hackney; Pierre Charbord; Brian P. Brunk; Christian J. Stoe...; Ihor R. Lemischka; Kateri A. Moore
• Keywords: Hematopoietic stem cell

The hematopoietic microenvironment provides a complex molecular milieu that regulates the self-renewal and differentiation activities of stem cells. We have characterized cell supportive stromal line, AFT024, was derived from murine fetal liver. Highly purified in vivo transplantable mouse cells are maintained AFT024 cultures at input levels, whereas other primitive progenitors expanded. In addition, human very effectively supported by AFT024. suggest line represents component an niche. To determine signals elaborated this niche, we undertook functional genomics approach combines extensive sequence mining subtracted cDNA library, high-density array hybridization in-depth bioinformatic analyses. data been assembled into biological process oriented database, represent profile candidate