Resolvin E1 is a pro-repair molecule that promotes intestinal epithelial wound healing

• DOI: 10.1073/pnas.1921335117
• Publication Date: 2020-04-16T23:44:36Z
• Authors: Miguel Quiros; Darius Feier; Dorothee Birkl; Rachit Agarwal; Dennis W. Zhou; Andrés J. García; Charles A. Parkos; Asma Nusrat
• Keywords: Intestinal mucosa

Resolution of intestinal inflammation and wound repair are active processes that mediate epithelial healing at mucosal surfaces. Lipid molecules referred to as specialized proresolving mediators (SPMs) play an important role in the restorative response. Resolvin E1 (RvE1), a SPM derived from omega-3 fatty acids, has been reported dampen by promoting anti-inflammatory responses including increased neutrophil spherocytosis macrophage production IL-10. Despite these observations, for RvE1 regulating cell migration proliferation during not explored. Using endoscopic biopsy-based model, we report is locally produced response injury. Exposure cells promoted increasing cellular through activation signaling pathways CREB, mTOR, Src-FAK. Additionally, RvE1-triggered small GTPase Rac1 led intracellular reactive oxygen species (ROS) production, cell-matrix adhesion, protrusions leading edge migrating cells. Furthermore, situ administration RvE1-encapsulated synthetic targeted polymeric nanoparticles into wounds repair. Together, findings demonstrate functions prorepair lipid mediator proliferation, highlight potential therapeutic applications this promote intestine.