Identification of a family of cAMP response element-binding protein coactivators by genome-scale functional analysis in mammalian cells

• DOI: 10.1073/pnas.1932773100
• Publication Date: 2003-10-14T19:05:46Z
• Authors: Vadim Iourgenko; Wenjun Zhang; Craig Mickanin; Ira Daly; Can Jiang; Jonathan M. Hexham; Anthony P. Orth; Loren Miraglia; Jodi Meltzer; Dan Garza; Gung-Wei Chirn; Elizabeth McWhinnie; Dalia Cohen; Joanne Skelton; Robert Terry; Yang Yu; Dale Bodian; Frank P. Buxton; Jian Zhu; Chuanzheng Song; Mark A. Labow
• Keywords: CREB1; Response element; Transcription; CREB-binding protein

This report describes an unbiased method for systematically determining gene function in mammalian cells. A total of 20,704 predicted human full-length cDNAs were tested induction the IL-8 promoter. number genes, including those cytokines, receptors, adapters, kinases, and transcription factors, identified that induced promoter through known regulatory sites. Proteins acted a cooperative interaction between AP-1 unrecognized cAMP response element (CRE)-like site also identified. protein, termed transducer regulated element-binding protein (CREB) (TORC1), was activated expression variant CRE consensus TORC1 potently CREB1 target bound CREB1, potent activation domain. functional Drosophila TORC Thus, TORCs represent family highly conserved CREB coactivators may control potency specificity CRE-mediated responses.