DIA-based systems biology approach unveils E3 ubiquitin ligase-dependent responses to a metabolic shift

• DOI: 10.1073/pnas.2020197117
• Publication Date: 2020-12-07T22:31:25Z
• Authors: Ozge Karayel; André C. Michaelis; Matthias Mann; Brenda A. Schulman; Christine R. Lang...
• Keywords: Proteome

Significance We use a single-run, data-independent acquisition–based mass spectrometry approach to generate and compare dozens of yeast proteomes in less than day, provide comprehensive resource detailing changes the proteome following commonly used stress treatments yeast. Our systems biology identifies validates regulatory targets an E3 ubiquitin ligase during metabolic switch, providing insights into interplay pathways. The speed, simplicity, scalability this workflow makes it particularly well-suited for screens any cellular system investigate specific effects deletions or mutants other perturbations obtain response biological on global level.