A versatile platform for locus-scale genome rewriting and verification

• DOI: 10.1073/pnas.2023952118
• Publication Date: 2021-03-01T21:38:10Z
• Authors: Ran Brosh; Jon M. Laurent; Raquel Ordoñez; Emily Huang; Megan S. Hogan; Angela M. Hitchcock; Leslie A. Mitchell; Sudarshan Pinglay; John A. Cadley; Raven D. Luther; David M. Truong; Jef D. Boeke; Matthew T. Maurano
• Keywords: Functional Genomics

Significance Functional analysis of noncoding genomic regulatory elements, which harbor the majority common human disease and trait associations, is complicated by their cellular context sensitivity. We developed Big-IN, a method for rewriting large segments mammalian genomes, including full genes surrounding elements. demonstrate flexible verification pipeline to identify correctly engineered cells. expect Big-IN will enable technologies synthesis assembly DNAs catalyze synthetic approach genomics.