Cell-free DNA profiling informs all major complications of hematopoietic cell transplantation

• DOI: 10.1073/pnas.2113476118
• Publication Date: 2022-01-20T22:00:57Z
• Authors: Alexandre Pellan ...; Matthew Pellan Cheng; Conor James Loy; Joan Sesing Lenz; Kaiwen Chen; Sami Smalling; Philip Burnham; Kaitlyn Marie Tim...; José Luis Orejas; Emily Silverman; Paz Polak; Francisco M. Marty; Jerome Ritz; Iwijn De Vlaminck
• Keywords: Cell-free fetal DNA

Significance Hematopoietic cell transplantation is the gold standard treatment for many blood disorders, including cancers. Nonetheless, frequent post-transplant complications limit long-term benefit of HCT. Here, we find that circulating cell-free DNA a highly versatile analyte monitoring most important HCT: Graft-Versus-Host Disease, infection, graft failure and disease relapse. We show these different therapeutic can be informed from single sequencing assay followed by disease-specific bioinformatic analyses. This test requires only low coverage compatible with small volumes blood. Cell-free may improve care allogeneic HCT recipients enabling earlier detection better prediction complex array occur after