The global RNA–RNA interactome ofKlebsiella pneumoniaeunveils a small RNA regulator of cell division

• DOI: 10.1073/pnas.2317322121
• Publication Date: 2024-02-20T18:55:21Z
• Authors: Eric Ruhland; Malte Siemers; Ruman Gerst; Felix Späth; Laura Nicole Vogt; Marc Thilo Figge; Kai Papenfort; Kathrin Sophie Fr...
• Keywords: Interactome; Repressor lexA; FtsZ; Transcription

The ubiquitous RNA chaperone Hfq is involved in the regulation of key biological processes many species across bacterial kingdom. In opportunistic human pathogen Klebsiella pneumoniae , deletion hfq gene affects global transcriptome, virulence, and stress resistance; however, ligands major RNA-binding protein this have remained elusive. study, we combined transcriptomic, co-immunoprecipitation, interactome analyses to compile an inventory conserved species-specific RNAs bound by monitor Hfq-mediated RNA–RNA interactions. addition dozens pairs, our study revealed Hfq-dependent small regulatory (sRNA), DinR, which processed from 3′ terminal portion dinI mRNA. Transcription controlled master regulator SOS response, LexA. As DinR accumulates K. response DNA damage, sRNA represses translation ftsZ transcript occupation ribosome binding site. Ectopic overexpression causes depletion mRNA inhibition cell division, while dinR antagonizes elongation presence damage. Collectively, work highlights important role RNA-based uncovers central LexA-controlled division during response.