MACSPI enables tissue-selective proteomic and interactomic analyses in multicellular organisms

• DOI: 10.1073/pnas.2319060121
• Publication Date: 2024-05-16T16:51:10Z
• Authors: Siyue Huang; Qiao Ran; Xiao-Meng Li; Xiucong Bao; Chaogu Zheng; Xiang David Li
• Keywords: Proteome; Interactome; Multicellular organism; Model Organism

Multicellular organisms are composed of many tissue types that have distinct morphologies and functions, which largely driven by specialized proteomes interactomes. To define the proteome interactome a specific type in an intact animal, we developed localized proteomics approach called Methionine Analog-based Cell-Specific Proteomics Interactomics (MACSPI). This method uses tissue-specific expression engineered methionyl-tRNA synthetase to label proteins with bifunctional amino acid 2-amino-5-diazirinylnonynoic selected cells. We applied MACSPI Caenorhabditis elegans, model multicellular organism, selectively label, capture, profile body wall muscle nervous system, led identification proteins. Using photo-cross-linker, successfully profiled HSP90 interactors muscles neurons identified stress-related interactors. Our study demonstrates can be used interactomes organisms.