PEPTIDYL TRANSFERS IN GRAMICIDIN S BIOSYNTHESIS FROM ENZYME-BOUND THIOESTER INTERMEDIATES

• DOI: 10.1073/pnas.63.4.1335
• Publication Date: 2006-05-31T06:14:52Z
• Authors: Wieland Gevers; Horst Kleinkauf; Fritz Lipmann
• Keywords: Thioester; Gramicidin S

The biosynthesis of the peptide antibiotic gramicidin S involves successive peptidyl transfer reactions between intermediates bound in thioester linkages to two active enzyme fractions, I and II. Fraction II activates recemizes phenylalanine, then initiates by catalyzing a reaction carboxyl group D-phenylalanine, an enzymic sulfhydryl group, free imino L-proline, one four L-amino acids all linked their functions separate groups on fraction I. Successive this type centers multienzyme complex lead formation thioester-bonded nascent chains and, ultimately, product.