Indirect Suppression of recB and recC Mutations by Exonuclease I Deficiency

• DOI: 10.1073/pnas.69.6.1366
• Publication Date: 2006-05-31T06:36:33Z
• Authors: Sidney R. Kushner; Haruko Nagaishi; Alvin J. Clark
• Keywords: RecBCD; Nuclease; Mitomycin C

recB - and recC strains of Escherichia coli K12 are recombination deficient sensitive to ultraviolet light the drug, mitomycin C. We have reported that sbcB mutations indirectly suppress all three phenotypes result in loss exonuclease I. In this publication we report occurrence other lead I UV sensitivity but not deficiency. These (called xonA ) cotransducible with his closely linked . Both mutant appear identical residual amounts nuclease activity on single-stranded DNA. It is hypothesized possesses a second than its known ability degrade DNA from 3′-OH terminus. Accordingly, would alter while not.