Role of DNA gyrase subunits in synthesis of bacteriophage phi X174 viral DNA.

• DOI: 10.1073/pnas.78.3.1532
• Publication Date: 2006-05-31T08:38:38Z
• Authors: R K Hamatake; R Mukai; M Hayashi
• Keywords: Novobiocin

The role of Escherichia coli DNA gyrase subunit A and B during phi X174 viral synthesis was investigated. Addition nalidixic acid (an inhibitor A) novobiocin B) to an in vitro system capable synthesizing inhibited synthesis. inhibition caused by novobiocin, however, not due specifically because composed extract containing novobiocin-resistant also novobiocin. requirement for the dispensability strand confirmed vivo examining host bacteria temperature-sensitive subunits.