Recombinant human gamma-interferon induces human monocyte polykaryon formation.

• DOI: 10.1073/pnas.81.14.4554
• Publication Date: 2006-05-31T09:27:08Z
• Authors: J B Weinberg; M M Hobbs; M A Misukonis
• Keywords: Monocyte

Monocyte or macrophage polykaryons (MP) are seen in different tissues various inflammatory states and normal bone (osteoclasts). The factors controlling the formation function of MP not completely understood. This study was designed to evaluate effects lymphokine gamma-interferon (IFN-gamma) on human monocyte vitro. Purified recombinant IFN-gamma [20-200 units/ml (0.1-1.0 nM)] caused appearance cultures monocytes cultured 10% unheated autologous serum. were noted by as early 36 hr culture with fusion indices 40%-60% up 160 nuclei per cell. effect both natural produced Staphylococcal enterotoxin A-stimulated lymphocytes, but IFN-alpha (leukocyte-derived recombinant) IFN-beta did induce formation. activity destroyed heating at 56 degrees C for 4 hr, incubating pH 2 3 antibody against IFN-gamma. Populations incubated days 100 units ml (0.5 nM) had enhanced capacity produce H2O2 response phorbol 12-myristate 13-acetate increased content acid phosphatase plasminogen activator. As determined autoradiography, incorporate [3H]dThd into their nuclei. Thus, appears a process fusion, "activate" monocytes, judged parameters.