DNA binding site for a factor(s) required to initiate simian virus 40 DNA replication.

• DOI: 10.1073/pnas.83.6.1646
• Publication Date: 2006-05-31T10:15:36Z
• Authors: M Yamaguchi; M L DePamphilis
• Keywords: Ter protein; DNA binding site; Origin recognition complex; Simian; Replication factor C

Efficient initiation of DNA replication in the absence nonspecific repair synthesis was obtained by using a modification system developed J.J. Li and T.J. Kelly [(1984) Proc. Natl. Acad. Sci. USA 81, 6973-6977]. Circular double-stranded plasmids replicated extracts CV-1 cells only when contained cis-acting origin sequence for simian virus 40 (ori) extract large tumor antigen. Competition between containing ori carrying deletions about served to identify that binds rate-limiting factor(s) required initiate replication. The minimum binding site (nucleotides 72-5243) encompassed one-half is (ori-core) plus contiguous on late gene side ori-core G + C-rich repeats facilitates (ori-auxiliary). This factor specific region, even though it excluded high-affinity antigen sites.