Secretion of follicle-stimulating hormone and production of inhibin are reciprocally related.

• DOI: 10.1073/pnas.84.13.4631
• Publication Date: 2006-05-31T10:20:49Z
• Authors: S Y Ying; J Czvik; A Becker; N Ling; N Ueno; R Guillemin
• Keywords: Gonadotropin

The production of inhibin in cultured granulosa cells from immature hypophysectomized, estrogen-treated rats and Sertoli normal animals was determined by a specific radioimmunoassay using an antiserum against synthetic replicate [Tyr30]inhibin alpha-chain-(1-30). amount immunoreactive detected the spent media these is proportion to density plated concentration exogenously added follicle-stimulating hormone (FSH). In presence estrogen precursor androstenedione (10(-7) M), FSH, but not luteinizing hormone, produced dose-dependent increase during 2-day culture cells. absence precursor, similar somewhat diminished responding FSH observed. Exogenously potentiated FSH-mediated release androstenedione. Neither nor estradiol had effect on production. A preparation pure isolated basis vitro bioassay characterized chemically specifically suppressed serum when it injected (24 micrograms per injection, two injections) into acutely ovariectomized rats. Thus, secreted suppresses secretion pituitary turn primarily responsible for gonadal cells, as classical negative-feedback relationship.