Alternative splicing of human elastin mRNA indicated by sequence analysis of cloned genomic and complementary DNA.

• DOI: 10.1073/pnas.84.16.5680
• Publication Date: 2006-05-31T10:23:37Z
• Authors: Z Indik; H Yeh; N Ornstein-Goldstein; P Sheppard; N Anderson; J C Rosenbloom; L Peltonen; J Rosenbloom
• Keywords: Tropoelastin; genomic DNA

Poly(A)+ RNA, isolated from a single 7-mo fetal human aorta, was used to synthesize cDNA by the RNase H method, and inserted into lambda gt10. Recombinant phage containing elastin sequences were identified hybridization with cloned, exon-containing fragments of gene. Three clones inserts 3.3, 2.7, 2.3 kilobases selected for further analysis. overlapping 17.8 gene also genomic libraries. Complete sequence analysis six demonstrated that: encompassed entire translated portion mRNA encoding 786 amino acids, including several unusual hydrophilic acid not previously in porcine tropoelastin, exons either hydrophobic or crosslinking domains protein alternated gene, great abundance Alu repetitive occurred throughout introns. The data indicated substantial alternative splicing mRNA. These results suggest potential significant variation precise molecular structure elastic fiber population.