A retrovirus carrying an MDR1 cDNA confers multidrug resistance and polarized expression of P-glycoprotein in MDCK cells.

• DOI: 10.1073/pnas.85.12.4486
• Publication Date: 2006-05-31T10:37:52Z
• Authors: I Pastan; M M Gottesman; K Ueda; E Lovelace; A V Rutherford; M C Willingham
• Keywords: P-glycoprotein

A full-length cDNA for the human multidrug resistance gene 1 (MDR1) has been inserted into a retroviral vector containing murine Harvey sarcoma virus from which viral oncogene was deleted. Ecotropic and amphotropic produced after transfection of this psi-2 PA-12 packaging cell lines. This conferred full phenotype on mouse Viral titers up to 2 X 10(5) drug-resistant colonies per ml were observed. Infected cells became resistant colchicine, vinblastine, doxorubicin, VP16 (etoposide), puromycin, but not cisplatin, indicating that presence MDR1 is sufficient cause resistance. When dog kidney line MDCK infected with virus, P-glycoprotein expressed in polarized manner upper surface cells, showing cloned also encodes information expression P-glycoprotein. The should be useful introducing drug variety types biological experiments vitro vivo.