Detection and mapping of spliced RNA from a human hepatoma cell line transfected with the hepatitis B virus genome.

• DOI: 10.1073/pnas.86.21.8422
• Publication Date: 2006-05-31T11:07:17Z
• Authors: T Suzuki; N Masui; K Kajino; I Saito; T Miyamura
• Keywords: Cosmid

HepG2 cells, known to support the replication and virion formation of hepatitis B virus (HBV), were transfected with a cosmid constructed contain 12 tandem head-to-tail repeats HBV genome for effective expression. We detected previously identified RNAs 3.3, 2.3, 2.0 kilobases (kb) that code core antigen, large surface middle/major respectively. also four additional 2.1, 1.7, 1.1, 0.7 kb [the lengths exclude poly(A) tail]. S1 mapping nucleotide sequencing data showed 2.1-kb RNA is spliced whose 5' 3' ends are identical those 3.3-kb RNA. The results suggest codes an altered antigen lacking last amino acid, cysteine, expression pregenomic regulated, at least in part, by splicing. map positions 1.7- 1.1-kb they carboxyl-terminal portions putative polymerase, whereas 0.7-kb X protein.