A second trans-spliced RNA leader sequence in the nematode Caenorhabditis elegans.

• DOI: 10.1073/pnas.86.22.8640
• Publication Date: 2006-05-31T11:07:52Z
• Authors: X Y Huang; D Hirsh
• Keywords: Trans-splicing; Primer extension

In the nematode Caenorhabditis elegans, 22-nucleotide RNA sequence called spliced leader (SL) is trans-spliced from 100-nucleotide-long SL to some mRNAs. We have identified a (SL2) whose differs that of original (SL1), although both are 22 nucleotides long. By primer-extension sequencing, SL2 but not SL1 was shown be present at 5' end mRNA encoded by one four glyceraldehyde-3-phosphate dehydrogenase genes. The other three genes encode mRNAs their ends. Therefore, trans-splicing process can discriminate transfer in gene-specific manner.