Major cold shock protein of Escherichia coli.

• DOI: 10.1073/pnas.87.1.283
• Publication Date: 2006-05-31T11:18:56Z
• Authors: J Goldstein; N S Pollitt; M Inouye
• Keywords: Cold-shock domain

When exponentially growing Escherichia coli cell cultures were transferred from 37 degrees C to 10 or 15 C, the production of a 7.4-kDa cytoplasmic protein (CS7.4) was prominently induced. The rate CS7.4 reached 13% total synthesis within 1-1.5 hr after shift and subsequently dropped lower basal level. Regulation expression very strict, such that undetectable at C. We have cloned gene encoding this completed nucleotide sequence analysis, which revealed encodes hydrophilic 70 amino acid residues.