Increased expression of eukaryotic translation initiation factors eIF-4E and eIF-2 alpha in response to growth induction by c-myc.

• DOI: 10.1073/pnas.90.13.6175
• Publication Date: 2006-05-31T12:25:50Z
• Authors: I B Rosenwald; D B Rhoads; L D Callanan; K J Isselbacher; E V Schmidt
• Keywords: Initiation factor; Transcription

Although activation of c-myc is a critical step in the development lymphomas and other tumors, its normal function(s) cell growth remain obscure because few myc-regulated genes are known. myc expression normally increases response to mitogens peaks G1 when additional protein synthesis required for cell-cycle progression. Protein controlled by availability translation initiation factors, including mRNA cap binding (eIF-4E) alpha subunit eIF-2 complex that binds initiator Met-tRNA. Consequently we examined eIF-4E evidence regulation c-myc. Expression correlated with fibroblasts after stimulation. In addition, was increased myc-transformed rat embryo but not ras-transformed cells. Transcription rates mRNAs were regulated cells expressing an estrogen receptor-Myc fusion protein. Finally, electrophoretic mobility-shift assays identified sequence element promoter, TCCGCAT-GCGCG, which specifically retarded extracts myc-expressing thought deregulate cancer activating transcription, suggesting specific should also function as oncogenes. previous studies these factors could induce neoplastic overexpression eIF-4E-transformed inhibition suppressor (eIF-2 kinase) caused malignant transformation. Our suggest one important biological may be increase increasing alpha.