RuvA and RuvB proteins of Escherichia coli exhibit DNA helicase activity in vitro.

• DOI: 10.1073/pnas.90.4.1315
• Publication Date: 2006-05-31T12:39:41Z
• Authors: I R Tsaneva; B Müller; S C West
• Keywords: Branch migration; Heteroduplex

The SOS-inducible ruvA and ruvB gene products of Escherichia coli are required for normal levels genetic recombination DNA repair. In vitro, RuvA protein interacts specifically with Holliday junctions and, together RuvB (an ATPase), promotes their movement along DNA. This process, known as branch migration, is important the formation heteroduplex this paper, we show that proteins promote unwinding partially duplex Using single-stranded circular substrates annealed fragments (52-558 nucleotides in length), strand displacement a 5'-->3' polarity. reaction ATP-dependent its efficiency inversely related to length These results genes encode helicase recognizes migration.