Reactive oxygen species and neutrophil respiratory burst cytochrome b558 are produced by kidney glomerular cells in passive Heymann nephritis.

• DOI: 10.1073/pnas.90.8.3645
• Publication Date: 2006-05-31T12:43:27Z
• Authors: T J Neale; R Ullrich; P Ojha; H Poczewski; A J Verhoeven; D Kerjaschki
• Keywords: Immunoelectron microscopy; Respiratory burst

Reactive oxygen species (ROS) have been implicated in the production of glomerular damage passive Heymann nephritis (PHN), an experimental form membranous nephropathy with neutrophil-independent proteinuria. Immunohistochemistry monoclonal antibodies specific for cytochrome b558 (a major component oxidoreductase complex respiratory burst stimulated neutrophilic granulocytes) showed that this enzyme is localized within visceral epithelial cells (GECs) a dense, granular pattern rats PHN and By immunoelectron-microscopy, was found membrane vesicles GEC also extracellularly on membranes facing basement (GBM). immunoblotting, detected highest concentration lysates isolated glomeruli from proteinuric rats. contrast, only traces were normal by immunohistochemistry. Depletion complement abolished expression cytochrome. Using ultrastructural cerium-H2O2 histochemistry technique, functional activity ROS-generating system demonstrated exclusively PHN, where H2O2 GBM. These results provide evidence proteinuria, GECs express externalize respiratory-burst enzymes generate ROS manner similar to granulocytes, which could then lead damage.