A tRNA-like structure is present in 10Sa RNA, a small stable RNA from Escherichia coli.

• DOI: 10.1073/pnas.91.20.9223
• Publication Date: 2006-05-31T12:56:09Z
• Authors: Y Komine; M Kitabatake; T Yokogawa; K Nishikawa; H Inokuchi
• Keywords: Nuclease protection assay

We have determined that 10Sa RNA (one of the small stable RNAs found in Escherichia coli) has an interesting structural feature: 5' end and 3' can be arranged a structure is equivalent to half-molecule (acceptor stem TFC stem-loop) alanine tRNA E. coli. Primer-extension analysis extracted from bacterial mutant with temperature-sensitive RNase P function revealed precursor (pre-10Sa RNA) folded into pre-tRNA-like vivo such it cleaved by generate mature RNA. The purified charged vitro. Disruption gene encoding (ssrA) caused reduction rate cell growth, which was especially apparent at 45 degrees C, motility on semisolid agar. These phenotypic characteristics deletion strain (delta ssrA) allowed us investigate effects some mutations vivo, although exact still remains unclear. When G.U pair (G3.U357) RNA, may determinant tRNA, changed G.A or G.C pair, ability complement delta ssrA lost. Furthermore, this inability phenotypes substitution bases could overcome introduction alanyl-tRNA synthetase (alaS) multicopy plasmid. evidence suggests proposed features are indeed manifested vivo.