Cre recombinase-mediated site-specific recombinationbetween plant chromosomes.

• DOI: 10.1073/pnas.91.5.1706
• Publication Date: 2006-05-31T13:05:56Z
• Authors: M Qin; C Bayley; T Stockton; D W Ow
• Keywords: Cre recombinase; Site-specific recombination; Cauliflower mosaic virus; FLP-FRT recombination; Cre-Lox recombination; Coding region

We report the use of bacteriophage P1 Cre-lox system for generating conservative site-specific recombination between tobacco chromosomes. Two constructs, one containing a promoterless hygromycin-resistance gene preceded by lox site (lox-hpt) and other cauliflower mosaic virus 35S promoter linked to sequence cre coding region (35S-lox-cre), were introduced separately into plants. Crosses plants harboring either construct produced with two constructs situated on different Plants events identified selecting hygromycin resistance, phenotype expressed upon recombination. Molecular analysis showed that these occurred specifically at sites resulted in reciprocal exchange flanking host DNA. Progenies 67-100% cotransmission new transgenes, 35S-lox-hpt lox-cre, consistent preferential cosegregation translocated These results illustrate systems can be useful tools large-scale manipulation eukaryotic chromosomes vivo.