Human CD8+ T lymphocyte subsets that express HLA class I-specific inhibitory receptors represent oligoclonally or monoclonally expanded cell populations.

• DOI: 10.1073/pnas.93.22.12433
• Publication Date: 2002-07-26T14:31:39Z
• Authors: M C Mingari; F Schiavetti; M Ponte; C Vitale; E Maggi; S Romagnani; J Demarest; G Pantaleo; A S Fauci; L Moretta
• Keywords: CD3 Complex; Receptors, Antigen, T-Cell, alpha-beta; Histocompatibility Antigens Class I; CD8-Positive T-Lymphocytes; Polymerase Chain Reaction; Recombinant Proteins; Cell Line; 3. Good health; Killer Cells, Natural; 03 medical and health sciences; Phenotype; 0302 clinical medicine; Humans; RNA, Messenger; Receptors, Immunologic

A small percentage of human T lymphocytes, predominantly CD8+ cells, express receptors for HLA class 1 molecules natural killer type (NK-R) that are inhibitory T-cell antigen receptor (TCR)-mediated functions. In the present study, it is demonstrated various NK-R typically expressed by NK cells also on periheral blood lymphocytes. These CD3+ NK-R+ have a cell surface phenotype typical memory as indicated expression CD45RO and CD29 lack CD28 CD45RA. Furthermore, combined use anti-TCR V beta-specific antibodies semiquantitative polymerase chain reaction assay, TCR repertoire in this subset was found to be skewed; fact, one or two beta families were largely represented, most other s barely detected. addition, analysis recombinant clones represented these oligoclonally monoclonally expanded.