Binding of factor VIIa to tissue factor induces alterations in gene expression in human fibroblast cells: Up-regulation of poly(A) polymerase

• DOI: 10.1073/pnas.94.23.12598
• Publication Date: 2002-07-26T14:40:52Z
• Authors: Usha R. Pendurthi; Deoraj Alok; L. Vijaya Mohan Rao
• Keywords: Northern blot; Factor VII

Tissue factor (TF) is the cellular receptor for an activated form of clotting VII (VIIa) and binding VII(a) to TF initiates coagulation cascade. Sequence structural patterns extracted from a global alignment confers homology with interferon receptors cytokine super family. Several recent studies suggested that could function as genuine signal transducing receptor. However, it unknown which biological function(s) cells are altered upon ligand, VIIa, TF. In present study, we examined effect VIIa cell surface on gene expression in fibroblasts. Differential mRNA display PCR technique was used identify transcriptional changes fibroblasts The showed either up or down-regulated several species. differential one such transcript, VIIa-induced up-regulation, confirmed by Northern blot analysis. Isolation full-length cDNA corresponding differentially expressed transcript revealed VIIa-up-regulated poly(A) polymerase. analysis various carcinomas normal human tissues over PAP cancer tissues. Enhanced tumor may potentially play important role metastasis.