Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase

• DOI: 10.1073/pnas.94.24.13087
• Publication Date: 2002-07-26T14:31:44Z
• Authors: Bevin P. Engelward; Geert Weeda; Michael D. Wyatt; José L. M. Broekhof; Jan de Wit; Ingrid Donker; James M. Allan; Barry Gold; Jan H. J. Hoeijma...; Leona D. Samson
• Keywords: MUTYH; Hypoxanthine

3-methyladenine (3MeA) DNA glycosylases remove 3MeAs from alkylated to initiate the base excision repair pathway. Here we report generation of mice deficient in 3MeA glycosylase encoded by Aag ( Mpg ) gene. Alkyladenine turns out be major not only for cytotoxic lesion, but also mutagenic 1, N 6 -ethenoadenine (ɛA) and hypoxanthine lesions. appears liver, testes, kidney, lung, ɛA kidney; another may expressed lung. Although alkyladenine has capacity 8-oxoguanine lesions, it does appear repair. Fibroblasts derived −/− are alkylation sensitive, indicating that similarly sensitive.