Disruption of overlapping transcripts in the ROSA βgeo 26 gene trap strain leads to widespread expression of β-galactosidase in mouse embryos and hematopoietic cells

• DOI: 10.1073/pnas.94.8.3789
• Publication Date: 2002-07-26T14:31:39Z
• Authors: Brian P. Zambrowicz; Akira Imamoto; Steve Fiering; Leonard A. Herzen...; William G. Kerr; Philippe Soriano
• Keywords: Chimera (genetics)

The ROSAβgeo26 (ROSA26) mouse strain was produced by random retroviral gene trapping in embryonic stem cells. Staining of ROSA26 tissues and fluorescence-activated cell sorter-Gal analysis hematopoietic cells demonstrates ubiquitous expression the proviral βgeo reporter gene, bone marrow transfer experiments illustrate general utility this for chimera transplantation studies. trap vector has integrated into a region that produces three transcripts. Two transcripts, lost homozygous animals, originate from common promoter share identical 5′ ends, but neither contains significant ORF. third transcript, originating reverse strand, shares antisense sequences with one noncoding This transcript potentially encodes novel protein at least 505 amino acids is conserved humans Caenorhabditis elegans .