IPF 2α -I: An index of lipid peroxidation in humans

• DOI: 10.1073/pnas.95.7.3449
• Publication Date: 2002-07-26T14:40:52Z
• Authors: Domenico Praticò; Orla P. Barry; John A. Lawson; Mustafa Adiyaman; Seong-Woo Hwang; Subhash P. Khanapure; Luigi Iuliano; Joshua Rokach; Garret A. FitzGerald
• Keywords: Isoprostanes; Prostaglandin F2alpha; Isoprostanes; Thromboxane B2

Isoprostanes are prostaglandin isomers produced from arachidonic acid by a free radical-catalyzed mechanism. Urinary excretion of 8- iso -prostaglandin F 2α , an isomer the PGG/H synthase (cyclooxygenase or COX) enzyme product, (PGF ), has exhibited promise as index oxidant stress in vivo. We have developed quantitative method to measure isoprostane -I, (IPF -I) class I (8- -PGF is IV), using gas chromatography/mass spectrometry. IPF -I severalfold abundant human urine iso- PGF with mean values 737 ± 20.6 pg/mg creatinine. Both isoprostanes formed radical-dependent manner low density lipoprotein oxidized copper vitro . However, unlike not COX-dependent platelets activated thrombin collagen Similarly, COX inhibition vivo no effect on -I. Neither serum cellular capacity generate isoprostane, nor urinary actual generation depressed aspirin indomethacin. In contrast, both thromboxane B 2 and its 11-dehydro metabolite inhibitors. Although formation substantially inhibitors, compound unaffected. elevated cigarette smokers compared controls (1525 180 versus 740 40 creatinine; P < 0.01) highly correlated ( r = 0.9; 0.001). novel lipid peroxidation which can be measured precision sensitivity. It -isoprostane radical- but manner. may minor product COX, this pathway contributes trivially, if at all, levels urine. smokers.