Auxin-induced K + channel expression represents an essential step in coleoptile growth and gravitropism

• DOI: 10.1073/pnas.96.21.12186
• Publication Date: 2002-07-26T14:38:21Z
• Authors: Katrin Philippar; Ines Fuchs; Hartwig Lüthen; Stefan Hoth; Claudia S. Bauer; Ken Haga; Gerhard Thiel; Karin Ljung; Göran Sandberg; Michael Böttger; Dirk Becker; Rainer Hedrich
• Keywords: 0303 health sciences; Potassium Channels; Indoleacetic Acids; Xenopus; Genes, Plant; Zea mays; Naphthaleneacetic Acids; Gravitropism; 03 medical and health sciences; Plant Growth Regulators; Gene Expression Regulation, Plant; Oocytes; Animals; Cotyledon; Plant Shoots

Auxin-induced growth of coleoptiles depends on the presence of potassium and is suppressed by K + channel blockers. To evaluate the role of K + channels in auxin-mediated growth, we isolated and functionally expressed ZMK1 and ZMK2 ( Zea mays K + channel 1 and 2), two potassium channels from maize coleoptiles. In growth experiments, the time course of auxin-induced expression of ZMK1 coincided with the kinetics of coleoptile elongation. Upon gravistimulation of maize seedlings, ZMK1 expression followed the gravitropic-induced auxin redistribution. K + channel expression increased even before a bending of the coleoptile was observed. The transcript level of ZMK2 , expressed in vascular tissue, was not affected by auxin. In patch-clamp studies on coleoptile protoplasts, auxin increased K + channel density while leaving channel properties unaffected. Thus, we conclude that coleoptile growth depends on the transcriptional up-regulation of ZMK1, an inwardly rectifying K + channel expressed in the nonvascular tissue of this organ.