PCGEM1, a prostate-specific gene, is overexpressed in prostate cancer

• DOI: 10.1073/pnas.97.22.12216
• Publication Date: 2002-07-26T14:36:44Z
• Authors: Vasantha Srikantan; Zhiqiang Zou; Gyorgy Petrovics; Linda Xu; Meena Augustus; Leland Davis; Jeffrey R. Livezey; Theresa Connell; Isabell A. Sester...; Kiyoshi Yoshino; Gregory S. Buzard; F. K. Mostofi; David G. McLeod; Judd W. Moul; Shiv Srivastava
• Keywords: Differential display

A prostate-specific gene, PCGEM1 , was identified by differential display analysis of paired normal and prostate cancer tissues. Multiple tissue Northern blot revealed that expressed exclusively in human tissue. Analysis expression matched primary tumor specimens tumor-associated overexpression 84% patients with situ hybridization assay 56% reverse transcription–PCR assay. Among various cell lines analyzed, detected only the androgen receptor-positive line LNCaP. Extensive DNA sequence cDNA genomic lacks protein-coding capacity suggests it may belong to an emerging class noncoding RNAs, also called “riboregulators.” The locus mapped chromosome 2q32. Taken together, remarkable prostate-tissue specificity androgen-dependent as well its elevated a significant percentage tissues suggest specific functions biology tumorigenesis gland.