Temporal Differences in the Phosphorylation State of Pre- and Postsynaptic Protein Kinase C Substrates B-50/GAP-43 and Neurogranin during Long Term Potentiation

• DOI: 10.1074/jbc.270.23.13892
• Publication Date: 2002-07-26T14:54:25Z
• Authors: Geert M.J. Ramakers; Pierre N.E. De Graan; Ivan J.A. Urban; Dick Kraay; Tong Tang; Piera Pasinelli; A. Beate Oestreicher; Willem H. Gispen
• Keywords: Neurogranin

The phosphorylation state of two identified neural-specific protein kinase C substrates (the presynaptic B-50 and the postsynaptic neurogranin) was monitored after induction long term potentiation in CA1 field rat hippocampus slices by quantitative immunoprecipitation following 32Pi labeling recording chamber. increased from 10 to 60 min, but no longer at 90 min had been induced, neurogranin only min. Increased not found when blocked with N-methyl-D-aspartate receptor antagonist D-2-amino-5-phosphonovalerate, low frequency stimulation applied or tetanic failed induce potentiation. Our data show that both are potentiation, thus providing strong evidence for pre- activation during narrow, partially overlapping, time windows